ABSTRACT
Objective:To establish an HPLC method for the determination of hesperidin, imperatorin, isoimperatorin, magnolol, honokiol and atractylodin in Huoxiang Zhengqi series preparations. Methods:An HPLC method was used with a Waters C18 chromato-graphic column (250 mm × 4. 6 mm, 5 μm). The mobile phase consisted of acetonitrile-0. 5% glacial acetic acid aqueous solution re-spectively for the mobile phase A and B with gradient elution. The flow rate was 1. 0ml·min-1;the detection wavelength was 290 nm;the column temperature was set at 40℃;the sample size was 10μl. Results:The linearity ranges of hesperdin, imperatorin, isoimper-atorin, magnolol, honokiol and atractylodin showed good linear relationships(r≥0.9993). The average recovery was 97.0%, 95. 0%,94. 3%,97. 6%,97. 3% and 93. 2%, respectively. The RSD was 2. 25%,2. 60%,2. 51%,1. 67%,1. 78% and 2. 50%( n=9), respectively. Conclusion:The method is simple and quick with good reproducibility, high precision and promising applicabili-ty, which can be used for the quality control of Huoxiang Zhengqi series preparations.
ABSTRACT
Objective:To establish an HPLC method for the determination of hesperidin, imperatorin, isoimperatorin, magnolol, honokiol and atractylodin in Huoxiang Zhengqi series preparations. Methods:An HPLC method was used with a Waters C18 chromato-graphic column (250 mm × 4. 6 mm, 5 μm). The mobile phase consisted of acetonitrile-0. 5% glacial acetic acid aqueous solution re-spectively for the mobile phase A and B with gradient elution. The flow rate was 1. 0ml·min-1;the detection wavelength was 290 nm;the column temperature was set at 40℃;the sample size was 10μl. Results:The linearity ranges of hesperdin, imperatorin, isoimper-atorin, magnolol, honokiol and atractylodin showed good linear relationships(r≥0.9993). The average recovery was 97.0%, 95. 0%,94. 3%,97. 6%,97. 3% and 93. 2%, respectively. The RSD was 2. 25%,2. 60%,2. 51%,1. 67%,1. 78% and 2. 50%( n=9), respectively. Conclusion:The method is simple and quick with good reproducibility, high precision and promising applicabili-ty, which can be used for the quality control of Huoxiang Zhengqi series preparations.
ABSTRACT
Objective:To establish a method for the determination of total chloride in oral rehydration salts powder (Ⅲ) by ion chromatography (IC). Methods:The analysis was performed on an IonPac AS 14A (250 mm × 4 mm) column with an IonPac AG 14A (50 mm × 4 mm) guard column and 9mmol·L-1 Na2 CO3 solution as the mobile phase. The flow rate was 1. 0 ml·min-1 with isocratic elution and the sample size was 25 μl. The suppressor was AERS 500(4mm) with 45mA suppression current, and an ECD was used for the detection. The quantity of chloride ion ( Cl-1 ) was calculated by the peak area with an external standard method. Re-sults:The linear range of Cl-1 was 0. 05-20. 00 mg·L-1(r=0. 9999);the recovery was 98. 89％(RSD=0. 56％, n=6). Conclu-sion:The results demonstrated that the method has the advantages of high sensitivity, simple sample pretreatment, promising accuracy and good reliability, which is suitable for the determination of total chloride in oral rehydration salt powder (Ⅲ) .
ABSTRACT
Objective:To establish the HPLC fingerprint of Qinghuo Zhimai tablets and determine the contents of geniposide,andrographolide and dehydroandrographolide.Methods:The analysis was performed on a CAPCELL PAK C18 column (250 mm×4.6 mm,5 μm) with acetonitrile and water as the mobile phase with gradient elution at the flow rate of 1.0 ml·min-1.The detection wavelength was 225 nm,and the column temperature was 40℃.Totally 13 batches of Qinghuo Zhimai tablets from different manufacturers were analyzed to establish the fingerprint of Qinghuo Zhimai tablets.Results:There were 14 common peaks calibrated,and the HPLC fingerprint common pattern was established for the HPLC fingerprint of Qinghuo Zhimai tablets.Using the method of reference substance attribution,3 compounds were determined as geniposide,andrographolide and dehydroandrographolide.The similarity of 9 batches in the 13 batches of Qinghuo Zhimai tablets was higher than 0.9.The content determination of geniposide,andrographolide and dehydroandrographolide showed good linearity (r>0.999 8,n=6),the average recovery was 96.90%,97.55% and 97.07% and RSDs were 1.61%,1.45% and 1.47%(n=9),respectively.Conclusion:The method is simple and accurate with good repeatability,which can be used for the identification and evaluation of Qinghuo Zhimai tablets,and provides important scientific basis for the quality control of Qinghuo Zhimai tablets.